Heme oxygenase 1 (HO-1) elisa kit - Database & Sql Blog Articles

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Heme oxygenase 1 (HO-1) elisa kit

Introduction to basic information

(Heme Oxygenase, HO) is the rate-limiting enzyme for heme degradation, converting heme to biliverdin IX, CO and iron, and biliverdin is then reduced to bilirubin. It is known that HO has three isozymes, HO-1, HO-2, HO-3.HO-2 and HO-3 are constitutively expressed in large amounts, and they may function as heme binding in normal cells. . While HO-1 is inducible, HO-1 is widely distributed in various mammalian tissue cells; HO-1 can be induced by various stimulating factors, such as oxidative stress, heat shock, ultraviolet irradiation, ischemia-reperfusion, and heavy metals. , bacterial lipopolysaccharide, cytokines and NO and its substrate heme. Recent research has focused on the biological effects of this enzymatic reaction product, which has antioxidant, anti-inflammatory, anti-apoptotic, signal transduction and immunomodulation and inhibition of adhesion molecule expression activity. The strong adaptive response of HO-1 to various stimulating factors reveals that it may play an important role in preventing inflammatory processes and oxidative tissue damage.

Heme oxygenase 1 (HO-1) elisa kit

Introduction to identification methods

Quantitative determination

The ELISA operation process is disorderly, and there are many factors affecting the reaction. In particular, the coating of the solid phase carrier is difficult to reach between the individual bodies. Therefore, in the quantitative measurement, each batch of tests must use a series of different concentrations of reference specifications. The norm curve is made under the same conditions. The ELISA kit measures the sandwich ELISA of large molecular weight substances. The scale of the normal curve is usually wide. The absorbance at the highest point of the curve can be close to 2.0. The semi-logarithmic paper is usually used for drawing. The concentration of the test object is the abscissa and the absorbance is vertical. Coordinates, the values ​​of each concentration are connected point by point, the resulting curve is usually S-shaped, the head and tail curves tend to be flat, and some of the central line is the most ideal inspection area.

Qualitative determination

The result of the qualitative determination is a simple answer to whether there is "yes" or "none" in the specimen to be tested, and "positive" or "negative" respectively. "Positive" indicates that the specimen has a response in the assay system. "Negative" is no response. Semi-quantitative results can also be obtained by qualitative discrimination, that is, the titer is used to indicate the intensity of the reaction, and its essence is still a qualitative experiment. ELISA kit In this semi-quantitative assay, the specimen is subjected to a series of dilutions and the highest dilution of the positive response is the titer. According to the level of the titer, it is possible to discriminate the strength of the specimen's reaction, which is more quantitative than the depth of the undiluted specimen.

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